In this nematode species, males are needed for reproduction, but not because of their genes

Biology

In the Mesorhabditis belari roundworm, the sole purpose of males is to help females produce clones of themselves. This unique form of reproduction was recently described by researchers from the CNRS, l’ENS de Lyon, l’Université Claude Bernard Lyon 1, and the Muséum national d'Histoire naturelle in an article published on 15 March 2019 in the journal Science.

In 1949, the young biologist Victor Nigon's doctoral thesis described the reproduction of various species of nematodes, small roundworms that live in the soil. These include Mesorhabditis belari, whose rare male specimens are required for reproduction, even though the genetic material found in sperm is rarely used by eggs. The resulting embryo produces a female, who is a clone of its mother. 

Seventy years later, this worm has once again drawn interest, this time from an international team led by researchers from the CNRS, l’ENS de Lyon, l’Université Claude Bernard Lyon 1, and the Muséum national d'Histoire naturelle1. They confirmed Victor Nigon’s initial observations, but also noted that in 9% of cases the embryo produced a male when the sperm genetic material was used after fertilization. Males can subsequently transmit their genes only to their sons, making M. belari a unique case in which males make no genetic contribution, and can instead be seen as a simple extension of females by helping them initiate the development of their eggs.

If males do not transmit the genes of their mothers, then at least they must help their mothers produce as many descendants as possible. This is possible only if the sons produced by a female help her daughters produce a large number of embryos. In other words, if the males preferentially fertilize their sisters.

But why would this lead to 9% males, instead of 2% or 20% for instance? By using “game theory,” researchers showed that producing 9% males is a stable evolutionary strategy, as this quantity is enough to ensure that a maximum number of female descendants are produced, without wasting too many resources in the production of males whose genes have no future.

Asexuality is a type of reproduction in which species consisting solely of females produce clones of themselves, as opposed to sexuality, in which individuals of the male sex enable genetic mixing with females. M. belari represents a new case, in which males can be useful to female reproduction, but with no genetic mixing. The research team will now explore how this type of reproduction emerged, and will test the long-term stability of the M. belari species through the study of its genome.

Two specimens of Mesorhabditis belari nematodes.
© Marie DELATTRE/LBMC/CNRS Photo library
Montage of fluorescent images of M. belari embryos as they divide. The cytoskeleton appears in green, female DNA in magenta, and male DNA in white. Male DNA is not included in the nucleus of embryonic cells as they undergo division.
© Marie DELATTRE/LBMC/CNRS Photo library

 

  • 1. In France, this research project was conducted by the Laboratoire de biologie et modélisation de la cellule (CNRS/ENS de Lyon/Université Claude Bernard Lyon 1), l’Institut de biologie de l'École normale supérieure (CNRS/ENS de Paris/Inserm), and l’Institut de systématique, évolution, biodiversité (CNRS/MNHN/EPHE/Sorbonne Université).
Bibliography

Males as somatic investment in a parthenogenetic nematode. Manon Grosmaire, Caroline Launay, Marion Siegwald, Thibault Brugière, Lilia Estrada-Virrueta, Duncan Berger, Claire Burny, Laurent Modolo, Mark Blaxter, Peter Meister, Marie-Anne Félix, Pierre-Henri Gouyon and Marie Delattre. Science, March 15, 2019. DOI : 10.1126/science.aau0099

Contact

Marie Delattre
CNRS researcher
François Maginiot
CNRS press officer